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recombinant human pad2  (Cayman Chemical)


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    Cayman Chemical recombinant human pad2
    Recombinant Human Pad2, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+pad2/gsk484/pm37648716-132-16-20
    Average 90 stars, based on 1 article reviews
    recombinant human pad2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Autoantibodies against citrullinated serum albumin in patients with rheumatoid arthritis
    Article Snippet: Recombinant human PAD4 and PAD2 were from Cayman Chemicals (Ann Arbor, MI).

    Article Title: Citrullinated fibrinogen-SAAs complex causes vascular metastagenesis.
    Article Snippet: A part of purified hFbgwas citrullinated by recombinant human PAD2 (Cayman Chemical, Ann Arbor,MI) in a deimination buffer (50mMHepes, pH 7.7, 10mMCaCl2, 5mM DTT) for 2 h at 37 °C.

    Article Title: Citrullinated fibrinogen-SAAs complex causes vascular metastagenesis
    Article Snippet: A part of purified hFbg was citrullinated by recombinant human PAD2 (Cayman Chemical, Ann Arbor, MI) in a deimination buffer (50 mM Hepes, pH 7.7, 10 mM CaCl 2 , 5 mM DTT) for 2 h at 37 °C.

    Article Title: Human cytomegalovirus-induced host protein citrullination is crucial for viral replication
    Article Snippet: In vitro citrullination of UL44 was performed as follows: 6 μM UL44∆C290 was incubated in PAD assay buffer (50 mM HEPES, pH 7.6, 10 mM CaCl 2 , 150 mM NaCl, 2 mM DTT, 12.5% glycerol for UL44) with recombinant human PAD2 or PAD4 (Chemical Cayman) at a concentration of 0.7 μM for 180 min, at 37 °C.

    Article Title: Applicability of Small-Molecule Inhibitors in the Study of Peptidyl Arginine Deiminase 2 (PAD2) and PAD4
    Article Snippet: Hereafter, wells were incubated with 30 ng/ml of recombinant human PAD2 (cat. 10785, Cayman Chemicals, Ann Arbor, MI, USA) or 150 ng/ml recombinant human PAD4 (cat. 10500, Cayman Chemicals) in citrullination buffer (100 mM Tris-HCl, 5 mM CaCl 2 , 1 mM DTT, pH 7.5) in the presence of increasing concentrations of the different PAD inhibitors (0.13–62.5 μM).

    Recombinant:

    Article Title: Identification of potential autoantigens in anti-CCP-positive and anti-CCP-negative rheumatoid arthritis using citrulline-specific protein arrays
    Article Snippet: .. Four slides were carefully transferred to different quadriperm chambers (Greiner BioOne, Kremsmünster, Austria) containing different citrulline reaction buffers consisting of 1.2 μg/mL recombinant human PAD2 or PAD4 (Cayman Chemicals, Ann Arbor, MI, USA); 1 mM 1,4-dithiothreitol (DTT), 10 mM CaCl, and 100 mM Tris–HCl and incubated at 37 °C for 3 h while shaking under horizontal rotation at 50 rpm (IKA, Germany, Königswinter). ..

    Article Title: Vimentin citrullination probed by a novel monoclonal antibody serves as a specific indicator for reactive astrocytes in neurodegeneration.
    Article Snippet: 1Ilsong Institute of Life Science,Hallym University, 15 Gwanpyeong-ro 170beon-gil, Anyang, Gyeonggi-do, 14066, Republic of Korea 2Department of Biomedical Gerontology, Graduate School of Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 24252, Republic of Korea 3Molecular Regulation of Aging, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan 4Department of Microbiology, College of Medicine, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 24252, Republic of Korea

    Article Title: Peptidylarginine deiminase 2 plays a key role in osteogenesis by enhancing RUNX2 stability through citrullination.
    Article Snippet: .. For in vitro citrullination assay, 0.5 μg recombinant human RUNX2 protein (rhRUNX2) (Origene) and one unit recombinant human PAD2 (#10785; Cayman Chemical, Ann Arbor, MI, USA) were incubated with reaction buffer (50mM HEPES (pH7.5), 5mM DTT, 10mM CaCl2, 50mM NaCl) at 37 °C for 2 h. After in vitro citrullination, the samples were labeled with Biotin-PG (Cayman) according to previously reported methods [25, 26]. ..

    Incubation:

    Article Title: Identification of potential autoantigens in anti-CCP-positive and anti-CCP-negative rheumatoid arthritis using citrulline-specific protein arrays
    Article Snippet: .. Four slides were carefully transferred to different quadriperm chambers (Greiner BioOne, Kremsmünster, Austria) containing different citrulline reaction buffers consisting of 1.2 μg/mL recombinant human PAD2 or PAD4 (Cayman Chemicals, Ann Arbor, MI, USA); 1 mM 1,4-dithiothreitol (DTT), 10 mM CaCl, and 100 mM Tris–HCl and incubated at 37 °C for 3 h while shaking under horizontal rotation at 50 rpm (IKA, Germany, Königswinter). ..

    Article Title: Vimentin citrullination probed by a novel monoclonal antibody serves as a specific indicator for reactive astrocytes in neurodegeneration.
    Article Snippet: 1Ilsong Institute of Life Science,Hallym University, 15 Gwanpyeong-ro 170beon-gil, Anyang, Gyeonggi-do, 14066, Republic of Korea 2Department of Biomedical Gerontology, Graduate School of Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 24252, Republic of Korea 3Molecular Regulation of Aging, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan 4Department of Microbiology, College of Medicine, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 24252, Republic of Korea

    Article Title: Peptidylarginine deiminase 2 plays a key role in osteogenesis by enhancing RUNX2 stability through citrullination.
    Article Snippet: .. For in vitro citrullination assay, 0.5 μg recombinant human RUNX2 protein (rhRUNX2) (Origene) and one unit recombinant human PAD2 (#10785; Cayman Chemical, Ann Arbor, MI, USA) were incubated with reaction buffer (50mM HEPES (pH7.5), 5mM DTT, 10mM CaCl2, 50mM NaCl) at 37 °C for 2 h. After in vitro citrullination, the samples were labeled with Biotin-PG (Cayman) according to previously reported methods [25, 26]. ..

    In Vitro:

    Article Title: Vimentin citrullination probed by a novel monoclonal antibody serves as a specific indicator for reactive astrocytes in neurodegeneration.
    Article Snippet: 1Ilsong Institute of Life Science,Hallym University, 15 Gwanpyeong-ro 170beon-gil, Anyang, Gyeonggi-do, 14066, Republic of Korea 2Department of Biomedical Gerontology, Graduate School of Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 24252, Republic of Korea 3Molecular Regulation of Aging, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan 4Department of Microbiology, College of Medicine, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 24252, Republic of Korea

    Article Title: Peptidylarginine deiminase 2 plays a key role in osteogenesis by enhancing RUNX2 stability through citrullination.
    Article Snippet: .. For in vitro citrullination assay, 0.5 μg recombinant human RUNX2 protein (rhRUNX2) (Origene) and one unit recombinant human PAD2 (#10785; Cayman Chemical, Ann Arbor, MI, USA) were incubated with reaction buffer (50mM HEPES (pH7.5), 5mM DTT, 10mM CaCl2, 50mM NaCl) at 37 °C for 2 h. After in vitro citrullination, the samples were labeled with Biotin-PG (Cayman) according to previously reported methods [25, 26]. ..

    Labeling:

    Article Title: Peptidylarginine deiminase 2 plays a key role in osteogenesis by enhancing RUNX2 stability through citrullination.
    Article Snippet: .. For in vitro citrullination assay, 0.5 μg recombinant human RUNX2 protein (rhRUNX2) (Origene) and one unit recombinant human PAD2 (#10785; Cayman Chemical, Ann Arbor, MI, USA) were incubated with reaction buffer (50mM HEPES (pH7.5), 5mM DTT, 10mM CaCl2, 50mM NaCl) at 37 °C for 2 h. After in vitro citrullination, the samples were labeled with Biotin-PG (Cayman) according to previously reported methods [25, 26]. ..



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    recombinant human pad2 (rhpad2)  (Cayman Chemical)
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    Cayman Chemical recombinant human pad2 (rhpad2)
    <t>PAD2</t> ribbon structure (PDB ID: 4N2C). The epitope 341-YLNRGDRWIQDEIEFGY-357 is shown in red, while calcium is shown in yellow. The three-dimensional structure of PAD2 was edited and prepared using UCSF chimera as described previously .
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    Image Search Results


    PAD2 ribbon structure (PDB ID: 4N2C). The epitope 341-YLNRGDRWIQDEIEFGY-357 is shown in red, while calcium is shown in yellow. The three-dimensional structure of PAD2 was edited and prepared using UCSF chimera as described previously .

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: PAD2 ribbon structure (PDB ID: 4N2C). The epitope 341-YLNRGDRWIQDEIEFGY-357 is shown in red, while calcium is shown in yellow. The three-dimensional structure of PAD2 was edited and prepared using UCSF chimera as described previously .

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques:

    Crossreactivity of chimeric anti-PAD2 antibodies with recombinant mouse PAD2. The values in the bracket indicates EC 50 values of antibodies against rhPAD2 and rmPAD2 enzymes. The enzyme-linked immunosorbent assay protocol is described .

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Crossreactivity of chimeric anti-PAD2 antibodies with recombinant mouse PAD2. The values in the bracket indicates EC 50 values of antibodies against rhPAD2 and rmPAD2 enzymes. The enzyme-linked immunosorbent assay protocol is described .

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

    Kinetics of binding of chimeric anti-PAD2 monoclonal antibodies (mAbs) with recombinant human PAD2.

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Kinetics of binding of chimeric anti-PAD2 monoclonal antibodies (mAbs) with recombinant human PAD2.

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Binding Assay, Bioprocessing, Recombinant

    Ability of anti-PAD2 antibodies to inhibit PAD2-mediated citrullination of N α -benzoyl-L-arginine ethyl ester (BAEE). S4, S10, S24, S108, S170, and S309 antibodies were incubated with recombinant human PAD2 (rhPAD2) and buffer (20 mM Tris-HCl (pH 7.6), 150 mM NaCl, and 1 mM dithiothreitol) in a total volume of 44 μ L for 30 min at 25°C. BAEE and CaCl 2 (volume: 6 μ L) were added simultaneously and the mixture was stirred well. The mixture comprised 5 nM rhPAD2, 10 mM BAEE, 1 mM CaCl 2 , and various concentrations of antibodies (0.1–600 nM). The mixture was incubated at 37°C for 4 h, and 12.5 μ L of 5 M perchloric acid was added to stop the reaction. The level of citrullinated BAEE was determined using a colorimetric assay system.

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Ability of anti-PAD2 antibodies to inhibit PAD2-mediated citrullination of N α -benzoyl-L-arginine ethyl ester (BAEE). S4, S10, S24, S108, S170, and S309 antibodies were incubated with recombinant human PAD2 (rhPAD2) and buffer (20 mM Tris-HCl (pH 7.6), 150 mM NaCl, and 1 mM dithiothreitol) in a total volume of 44 μ L for 30 min at 25°C. BAEE and CaCl 2 (volume: 6 μ L) were added simultaneously and the mixture was stirred well. The mixture comprised 5 nM rhPAD2, 10 mM BAEE, 1 mM CaCl 2 , and various concentrations of antibodies (0.1–600 nM). The mixture was incubated at 37°C for 4 h, and 12.5 μ L of 5 M perchloric acid was added to stop the reaction. The level of citrullinated BAEE was determined using a colorimetric assay system.

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Incubation, Recombinant, Colorimetric Assay

    Ability of anti-PAD2 antibodies to inhibit recombinant human PAD2- (rhPAD2-) mediated histone citrullination. The anti-PAD2 antibodies were mixed with rhPAD2 and buffer as described in the legend of at 25°C for 30 min. Histone (1.8 mg/mL) and CaCl 2 (1 mM) were added simultaneously and the mixture was incubated for 4 h at 37°C. Citrullinated histone was determined using a colorimetric assay.

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Ability of anti-PAD2 antibodies to inhibit recombinant human PAD2- (rhPAD2-) mediated histone citrullination. The anti-PAD2 antibodies were mixed with rhPAD2 and buffer as described in the legend of at 25°C for 30 min. Histone (1.8 mg/mL) and CaCl 2 (1 mM) were added simultaneously and the mixture was incubated for 4 h at 37°C. Citrullinated histone was determined using a colorimetric assay.

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Recombinant, Incubation, Colorimetric Assay

    Alanine scanning of epitopes along with their half-maximal effective concentration. EC 50 (ng/mL) values determined using enzyme-linked immunosorbent assay ∗ .

    Journal: Journal of Immunology Research

    Article Title: Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

    doi: 10.1155/2021/6659960

    Figure Lengend Snippet: Alanine scanning of epitopes along with their half-maximal effective concentration. EC 50 (ng/mL) values determined using enzyme-linked immunosorbent assay ∗ .

    Article Snippet: Recombinant human PAD2 (rhPAD2) was purchased from Cayman Chemical (Michigan, USA) and was used for all experiments unless otherwise stated.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Residue